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Identification and Expression of G Protein-Coupled Receptors edited
by Kevin R. Lynch The past decade has seen tremendous advances in
the study of G protein-coupled receptors (GPCRs), including the
molecular cloning and identification of more than 100 GPCR genes.
But while GPCRs serve as targets for more than 300 medicines in the
modern pharmacopoeia, the shrinking pool of known ligands and the
continuing discovery of orphan GPCR genes have underscored the need
for new approaches to ligand identification. Identification and
Expression of G Protein-Coupled Receptors addresses this new
direction in GPCR biochemistry-offering a definitive laboratory
bench manual that emphasizes expression over primary cloning
strategies. In a series of expert contributions by well-known
researchers, this book provides detailed protocols for various
expression systems-from bacteria to mammalian cells-as well as
straightforward opinions on the advantages and shortcomings of each
approach. Topics covered include:
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